Windows 10 Home

Python 3.7.3 [MSC v.1916 64 bit (AMD64)] on win32

I open the file so etc.:

settings_file = open('settings.txt','r')
settings = [line.strip() for line in settings_file.readlines()]
# Filter out comments and empties
settings = [line for line in settings if (line!='' and line[0] != '#')]

I attach the .txt file.

I think the problem can be because of that line, because, before windows update, I used this .txt file and the first 4 parts of its content has been used to begin the program, but now, it sees that as None. The problem is with my new folders, when I create a new folder. It works different there, but in the old folders, it works as before.