Ahh okay.  I've reproduced it in trunk at changeset 053bc5ca199b.

As suggested, I ran: PCBuild\pythonw.exe lib\idlelib\idle.py

Python 3.3a0 (default, Apr  2 2011, 21:55:40) [MSC v.1500 32 bit (Intel)] on win32
Type "copyright", "credits" or "license()" for more information.
>>> import subprocess
>>> subprocess.Popen(["python", "-c", "print(32)"], stdin=None, stdout=subprocess.PIPE, stderr=subprocess.PIPE)
Traceback (most recent call last):
  File "<pyshell#1>", line 1, in <module>
    subprocess.Popen(["python", "-c", "print(32)"], stdin=None, stdout=subprocess.PIPE, stderr=subprocess.PIPE)
  File "C:\dev\cpython\cpython\lib\subprocess.py", line 732, in __init__
    errread, errwrite) = self._get_handles(stdin, stdout, stderr)
  File "C:\dev\cpython\cpython\lib\subprocess.py", line 903, in _get_handles
    p2cread = self._make_inheritable(p2cread)
  File "C:\dev\cpython\cpython\lib\subprocess.py", line 946, in _make_inheritable
    _subprocess.DUPLICATE_SAME_ACCESS)
WindowsError: [Error 6] The handle is invalid
>>> 

So, the issue is definitely not fixed.