diff --git a/Lib/test/script_helper.py b/Lib/test/script_helper.py
--- a/Lib/test/script_helper.py
+++ b/Lib/test/script_helper.py
@@ -78,7 +78,7 @@ def assert_python_failure(*args, **env_v
     """
     return _assert_python(False, *args, **env_vars)
 
-def spawn_python(*args, **kw):
+def spawn_python(*args, stdout=subprocess.PIPE, stderr=subprocess.STDOUT, **kw):
     """Run a Python subprocess with the given arguments.
 
     kw is extra keyword args to pass to subprocess.Popen. Returns a Popen
@@ -87,7 +87,7 @@ def spawn_python(*args, **kw):
     cmd_line = [sys.executable, '-E']
     cmd_line.extend(args)
     return subprocess.Popen(cmd_line, stdin=subprocess.PIPE,
-                            stdout=subprocess.PIPE, stderr=subprocess.STDOUT,
+                            stdout=stdout, stderr=stderr,
                             **kw)
 
 def kill_python(p):
diff --git a/Lib/test/test_cmd_line_script.py b/Lib/test/test_cmd_line_script.py
--- a/Lib/test/test_cmd_line_script.py
+++ b/Lib/test/test_cmd_line_script.py
@@ -1,5 +1,6 @@
 # tests command line execution of scripts
 
+import contextlib
 import importlib
 import importlib.machinery
 import zipimport
@@ -8,6 +9,7 @@ import sys
 import os
 import os.path
 import py_compile
+import subprocess
 
 import textwrap
 from test import support
@@ -173,6 +175,53 @@ class CmdLineTest(unittest.TestCase):
         expected = repr(importlib.machinery.BuiltinImporter).encode("utf-8")
         self.assertIn(expected, out)
 
+    @contextlib.contextmanager
+    def interactive_python(self, separate_stderr=False):
+        if separate_stderr:
+            p = spawn_python('-i', bufsize=1, stderr=subprocess.PIPE)
+            stderr = p.stderr
+        else:
+            p = spawn_python('-i', bufsize=1, stderr=subprocess.STDOUT)
+            stderr = p.stdout
+        try:
+            # Drain stderr until prompt
+            while True:
+                data = stderr.read(4)
+                if data == b">>> ":
+                    break
+                stderr.readline()
+            yield p
+        finally:
+            kill_python(p)
+            stderr.close()
+
+    def check_repl_stdout_flush(self, separate_stderr=False):
+        with self.interactive_python(separate_stderr) as p:
+            p.stdin.write(b"print('foo')\n")
+            p.stdin.flush()
+            self.assertEqual(b'foo', p.stdout.readline().strip())
+
+    def check_repl_stderr_flush(self, separate_stderr=False):
+        with self.interactive_python(separate_stderr) as p:
+            p.stdin.write(b"1/0\n")
+            p.stdin.flush()
+            stderr = p.stderr if separate_stderr else p.stdout
+            self.assertIn(b'Traceback ', stderr.readline())
+            self.assertIn(b'File "<stdin>"', stderr.readline())
+            self.assertIn(b'ZeroDivisionError', stderr.readline())
+
+    def test_repl_stdout_flush(self):
+        self.check_repl_stdout_flush()
+
+    def test_repl_stdout_flush_separate_stderr(self):
+        self.check_repl_stdout_flush(True)
+
+    def test_repl_stderr_flush(self):
+        self.check_repl_stderr_flush()
+
+    def test_repl_stderr_flush_separate_stderr(self):
+        self.check_repl_stderr_flush(True)
+
     def test_basic_script(self):
         with temp_dir() as script_dir:
             script_name = _make_test_script(script_dir, 'script')
diff --git a/Python/pythonrun.c b/Python/pythonrun.c
--- a/Python/pythonrun.c
+++ b/Python/pythonrun.c
@@ -1444,12 +1444,13 @@ PyRun_InteractiveOneObject(FILE *fp, PyO
     d = PyModule_GetDict(m);
     v = run_mod(mod, filename, d, d, flags, arena);
     PyArena_Free(arena);
-    flush_io();
     if (v == NULL) {
         PyErr_Print();
+        flush_io();
         return -1;
     }
     Py_DECREF(v);
+    flush_io();
     return 0;
 }