diff --git a/Lib/test/test_tcl.py b/Lib/test/test_tcl.py
--- a/Lib/test/test_tcl.py
+++ b/Lib/test/test_tcl.py
@@ -8,6 +8,9 @@
 # Skip this test if the _tkinter module wasn't built.
 _tkinter = test_support.import_module('_tkinter')
 
+# Make sure Tkinter._fix runs to set up the environment
+test_support.import_fresh_module('Tkinter')
+
 from Tkinter import Tcl
 from _tkinter import TclError
 
diff --git a/Lib/test/test_tk.py b/Lib/test/test_tk.py
--- a/Lib/test/test_tk.py
+++ b/Lib/test/test_tk.py
@@ -4,6 +4,9 @@
 # Skip test if _tkinter wasn't built.
 test_support.import_module('_tkinter')
 
+# Make sure Tkinter._fix runs to set up the environment
+test_support.import_fresh_module('Tkinter')
+
 this_dir = os.path.dirname(os.path.abspath(__file__))
 lib_tk_test = os.path.abspath(os.path.join(this_dir, os.path.pardir,
     'lib-tk', 'test'))
diff --git a/Lib/test/test_ttk_guionly.py b/Lib/test/test_ttk_guionly.py
--- a/Lib/test/test_ttk_guionly.py
+++ b/Lib/test/test_ttk_guionly.py
@@ -5,6 +5,9 @@
 # Skip this test if _tkinter wasn't built.
 test_support.import_module('_tkinter')
 
+# Make sure Tkinter._fix runs to set up the environment
+test_support.import_fresh_module('Tkinter')
+
 this_dir = os.path.dirname(os.path.abspath(__file__))
 lib_tk_test = os.path.abspath(os.path.join(this_dir, os.path.pardir,
     'lib-tk', 'test'))
diff --git a/Lib/test/test_ttk_textonly.py b/Lib/test/test_ttk_textonly.py
--- a/Lib/test/test_ttk_textonly.py
+++ b/Lib/test/test_ttk_textonly.py
@@ -4,6 +4,9 @@
 # Skip this test if _tkinter does not exist.
 test_support.import_module('_tkinter')
 
+# Make sure Tkinter._fix runs to set up the environment
+test_support.import_fresh_module('Tkinter')
+
 this_dir = os.path.dirname(os.path.abspath(__file__))
 lib_tk_test = os.path.abspath(os.path.join(this_dir, '..', 'lib-tk', 'test'))