from sys import path
import winsound
import time

# start playing a sound
winsound.PlaySound(path[0] + '/sound.wav', winsound.SND_FILENAME)

# wait for a moment
time.sleep(0.1)

# stop it as described in the documentation
winsound.PlaySound(path[0] + '/sound.wav', winsound.SND_PURGE)

# stop it as done in a test file I found
winsound.PlaySound(None, winsound.SND_PURGE)